Reproduction Abstracts

Introduction: Oocytes that are not fertilized in a specific time-frame after ovulation undergo a time-dependent process of degradation and loss of developmental potential that can be referred to as postovulatory ageing. This process occurs in vivo and under specific in vitro settings. Here, we studied epigenetic changes related to postovulatory ageing.Materials and methods: Bovine oocytes were collected from slaughterhouse ovaries and m...

Introduction: Induced pluripotent stem (iPS) cells are considered as a seminal breakthrough in stem cell research and are promising for the development of advanced regenerative therapies. Considering the potential of this technology for both basic and clinical research, it is tempting to extend this research to important livestock species, such as cattle, in which ES cell lines are yet not available.Materials and methods: Here, we describe a non-viral me...

Introduction: Lipid content of oocytes and early embryos is critical for embryonic and fetal development. Indeed, the success of assisted reproductive techniques (ARTs) such as cryopreservation and in vitro embryo production is heavily affected by the lipid content of preimplantation embryos.Materials and methods: Lipid profiling of single in vitro (IVTB) and in vivo (IVVB) produced bovine embryos was performed by desorption el...